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hdac assay developer  (BPS Bioscience)


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    BPS Bioscience hdac assay developer
    Hdac Assay Developer, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+hdac10/HDAC10%2C+FLAG-tag+Recombinant/pm41109068-447-15-18
    Average 94 stars, based on 27 article reviews
    hdac assay developer - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Targeting Relevant HDACs to Support the Survival of Cone Photoreceptors in Inherited Retinal Diseases: Identification of a Potent Pharmacological Tool with In Vitro and In Vivo Efficacy.
    Article Snippet: Inherited retinal diseases, which include retinitis pigmentosa, are a family of genetic disorders characterized by gradual rod-cone degeneration and vision loss, without effective pharmacological treatments.. Experimental approaches aim to delay disease progression, supporting cones’ survival, crucial for human vision.. Histone deacetylases (HDACs) mediate the activation of epigenetic and nonepigenetic pathways that modulate cone degeneration in RP mouse models.

    Article Title: A novel potent class I HDAC inhibitor reverses the STAT4/p66Shc apoptotic defect in B cells from chronic lymphocytic leukemia patients.
    Article Snippet: .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2 A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Reversing TGF-β1-induced fibrotic phenotype in human lung fibroblasts using a PROTAC tool derived from an indoline-based HDAC6 inhibitor
    Article Snippet: Images were taken using a confocal microscope (Zeiss LSM700; Zeiss GmbH, Oberkochen, Germany). .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. #50040) for HDAC8 assays.

    Article Title: Pioneering first-in-class FAAH-HDAC inhibitors as potential multitarget neuroprotective agents.
    Article Snippet: .. 4.3.1 | Enzymatic assays on human HDACs For the evaluation of their inhibitory activity, different concentrations of the novel compounds were incubated in a low‐binding black 96‐well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25mM Tris/HCl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, and 0.1mg/mL bovine serum albumin for 30min at 37°C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Spirotetrahydroisoquinoline-Based Histone Deacetylase Inhibitors as New Antifibrotic Agents: Biological Evaluation in Human Fibroblasts from Bronchoalveolar Lavages of Idiopathic Pulmonary Fibrosis Patients.
    Article Snippet: .. Human HDAC inhibition assays For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), 4ng of human recombinant HDAC3 (BPS Bioscience, Cat. # 50003), 6 ng of human recombinant HDAC5 (BPS Bioscience, Cat. # 50005) or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) or 100 ng human recombinant HDAC11 (BPS Bioscience, Cat. # 50021) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, HDAC3 and HDAC10 assays, or of the fluorogenic HDAC substrate class IIA (BPS Bioscience; Cat. # 50040) for HDAC5, HDAC8 and HDAC11 assays.

    Article Title: New Vanillyl-capped HDAC inhibitors exhibit anti-tumor efficacy in neuroblastoma and glioblastoma cells.
    Article Snippet: Histone deacetylases 6 and 8 (HDAC6/8) have emerged as promising therapeutic targets in aggressive neural tumors such as neuroblastoma and glioblastoma.. Herein, we report the design, synthesis, and comprehensive biological evaluation of a novel series of hydroxamic acid-based inhibitors (5a–p), featuring nature-inspired vanillyl CAP groups.. Structure–activity relationship (SAR) analysis, supported by molecular docking, elucidated the role of CAP, connecting unit, and linker structure, alongside zinc-binding group orientation, on isoform selectivity and potency.

    Incubation:

    Article Title: Targeting Relevant HDACs to Support the Survival of Cone Photoreceptors in Inherited Retinal Diseases: Identification of a Potent Pharmacological Tool with In Vitro and In Vivo Efficacy.
    Article Snippet: Inherited retinal diseases, which include retinitis pigmentosa, are a family of genetic disorders characterized by gradual rod-cone degeneration and vision loss, without effective pharmacological treatments.. Experimental approaches aim to delay disease progression, supporting cones’ survival, crucial for human vision.. Histone deacetylases (HDACs) mediate the activation of epigenetic and nonepigenetic pathways that modulate cone degeneration in RP mouse models.

    Article Title: A novel potent class I HDAC inhibitor reverses the STAT4/p66Shc apoptotic defect in B cells from chronic lymphocytic leukemia patients.
    Article Snippet: .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2 A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Reversing TGF-β1-induced fibrotic phenotype in human lung fibroblasts using a PROTAC tool derived from an indoline-based HDAC6 inhibitor
    Article Snippet: Images were taken using a confocal microscope (Zeiss LSM700; Zeiss GmbH, Oberkochen, Germany). .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. #50040) for HDAC8 assays.

    Article Title: Pioneering first-in-class FAAH-HDAC inhibitors as potential multitarget neuroprotective agents.
    Article Snippet: .. 4.3.1 | Enzymatic assays on human HDACs For the evaluation of their inhibitory activity, different concentrations of the novel compounds were incubated in a low‐binding black 96‐well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25mM Tris/HCl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, and 0.1mg/mL bovine serum albumin for 30min at 37°C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Synthesis and Biological Evaluation of Novel 2-Aroyl Benzofuran-Based Hydroxamic Acids as Antimicrotubule Agents.
    Article Snippet: .. Different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for the HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for the HDAC8 assays.

    Article Title: Synthesis and Biological Evaluation of Novel 2-Aroyl Benzofuran-Based Hydroxamic Acids as Antimicrotubule Agents
    Article Snippet: .. Different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for the HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for the HDAC8 assays.

    Article Title: Spirotetrahydroisoquinoline-Based Histone Deacetylase Inhibitors as New Antifibrotic Agents: Biological Evaluation in Human Fibroblasts from Bronchoalveolar Lavages of Idiopathic Pulmonary Fibrosis Patients.
    Article Snippet: .. Human HDAC inhibition assays For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), 4ng of human recombinant HDAC3 (BPS Bioscience, Cat. # 50003), 6 ng of human recombinant HDAC5 (BPS Bioscience, Cat. # 50005) or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) or 100 ng human recombinant HDAC11 (BPS Bioscience, Cat. # 50021) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, HDAC3 and HDAC10 assays, or of the fluorogenic HDAC substrate class IIA (BPS Bioscience; Cat. # 50040) for HDAC5, HDAC8 and HDAC11 assays.

    Article Title: New Vanillyl-capped HDAC inhibitors exhibit anti-tumor efficacy in neuroblastoma and glioblastoma cells.
    Article Snippet: Histone deacetylases 6 and 8 (HDAC6/8) have emerged as promising therapeutic targets in aggressive neural tumors such as neuroblastoma and glioblastoma.. Herein, we report the design, synthesis, and comprehensive biological evaluation of a novel series of hydroxamic acid-based inhibitors (5a–p), featuring nature-inspired vanillyl CAP groups.. Structure–activity relationship (SAR) analysis, supported by molecular docking, elucidated the role of CAP, connecting unit, and linker structure, alongside zinc-binding group orientation, on isoform selectivity and potency.

    Recombinant:

    Article Title: Targeting Relevant HDACs to Support the Survival of Cone Photoreceptors in Inherited Retinal Diseases: Identification of a Potent Pharmacological Tool with In Vitro and In Vivo Efficacy.
    Article Snippet: Inherited retinal diseases, which include retinitis pigmentosa, are a family of genetic disorders characterized by gradual rod-cone degeneration and vision loss, without effective pharmacological treatments.. Experimental approaches aim to delay disease progression, supporting cones’ survival, crucial for human vision.. Histone deacetylases (HDACs) mediate the activation of epigenetic and nonepigenetic pathways that modulate cone degeneration in RP mouse models.

    Article Title: A novel potent class I HDAC inhibitor reverses the STAT4/p66Shc apoptotic defect in B cells from chronic lymphocytic leukemia patients.
    Article Snippet: .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2 A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Reversing TGF-β1-induced fibrotic phenotype in human lung fibroblasts using a PROTAC tool derived from an indoline-based HDAC6 inhibitor
    Article Snippet: Images were taken using a confocal microscope (Zeiss LSM700; Zeiss GmbH, Oberkochen, Germany). .. For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1 and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. #50040) for HDAC8 assays.

    Article Title: Pioneering first-in-class FAAH-HDAC inhibitors as potential multitarget neuroprotective agents.
    Article Snippet: .. 4.3.1 | Enzymatic assays on human HDACs For the evaluation of their inhibitory activity, different concentrations of the novel compounds were incubated in a low‐binding black 96‐well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer composed of 25mM Tris/HCl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, and 0.1mg/mL bovine serum albumin for 30min at 37°C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for HDAC8 assays.

    Article Title: Synthesis and Biological Evaluation of Novel 2-Aroyl Benzofuran-Based Hydroxamic Acids as Antimicrotubule Agents.
    Article Snippet: .. Different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 ◦C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for the HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for the HDAC8 assays.

    Article Title: Synthesis and Biological Evaluation of Novel 2-Aroyl Benzofuran-Based Hydroxamic Acids as Antimicrotubule Agents
    Article Snippet: .. Different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) in an assay buffer containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for the HDAC6, HDAC1, and HDAC10 assays, or of the fluorogenic HDAC substrate class 2A (BPS Bioscience; Cat. # 50040) for the HDAC8 assays.

    Article Title: Spirotetrahydroisoquinoline-Based Histone Deacetylase Inhibitors as New Antifibrotic Agents: Biological Evaluation in Human Fibroblasts from Bronchoalveolar Lavages of Idiopathic Pulmonary Fibrosis Patients.
    Article Snippet: .. Human HDAC inhibition assays For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), 4ng of human recombinant HDAC3 (BPS Bioscience, Cat. # 50003), 6 ng of human recombinant HDAC5 (BPS Bioscience, Cat. # 50005) or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) or 100 ng human recombinant HDAC11 (BPS Bioscience, Cat. # 50021) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, HDAC3 and HDAC10 assays, or of the fluorogenic HDAC substrate class IIA (BPS Bioscience; Cat. # 50040) for HDAC5, HDAC8 and HDAC11 assays.

    Article Title: New Vanillyl-capped HDAC inhibitors exhibit anti-tumor efficacy in neuroblastoma and glioblastoma cells.
    Article Snippet: Histone deacetylases 6 and 8 (HDAC6/8) have emerged as promising therapeutic targets in aggressive neural tumors such as neuroblastoma and glioblastoma.. Herein, we report the design, synthesis, and comprehensive biological evaluation of a novel series of hydroxamic acid-based inhibitors (5a–p), featuring nature-inspired vanillyl CAP groups.. Structure–activity relationship (SAR) analysis, supported by molecular docking, elucidated the role of CAP, connecting unit, and linker structure, alongside zinc-binding group orientation, on isoform selectivity and potency.

    Inhibition:

    Article Title: Spirotetrahydroisoquinoline-Based Histone Deacetylase Inhibitors as New Antifibrotic Agents: Biological Evaluation in Human Fibroblasts from Bronchoalveolar Lavages of Idiopathic Pulmonary Fibrosis Patients.
    Article Snippet: .. Human HDAC inhibition assays For the evaluation of their inhibitory activity, different concentrations of the compounds were incubated in a low-binding black 96-well plate with 30 ng of human recombinant HDAC6 (BPS Bioscience, San Diego, CA, USA; Cat. # 50056), human recombinant HDAC1 (BPS Bioscience; Cat. # 50051), human recombinant HDAC8 (BPS Bioscience; Cat. # 50008), 4ng of human recombinant HDAC3 (BPS Bioscience, Cat. # 50003), 6 ng of human recombinant HDAC5 (BPS Bioscience, Cat. # 50005) or 500 ng of human recombinant HDAC10 (BPS Bioscience; Cat. # 50060) or 100 ng human recombinant HDAC11 (BPS Bioscience, Cat. # 50021) in an assay buffer composed of 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/mL bovine serum albumin for 30 min at 37 °C. .. At the end of the incubation, the deacetylation reaction was initiated by adding 200 μM of the fluorogenic acetylated HDAC substrate 3 (BPS Bioscience; Cat. # 50037) for HDAC6, HDAC1, HDAC3 and HDAC10 assays, or of the fluorogenic HDAC substrate class IIA (BPS Bioscience; Cat. # 50040) for HDAC5, HDAC8 and HDAC11 assays.



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